The role of Odoroside A in immune transformation in RAW264.7 cells

Authors : Fatma Seçer Çelik, Canan Eroğlu Güneş

Pages : 534-540

Doi:10.31362/patd.1433389

View : 69 | Download : 93

Publication Date : 2024-07-05

Article Type : Research Paper

Abstract :Purpose: Macrophages are one of the most important elements of the immune system and play dual role in inflammation. They regulate both the formation of the immune response in the environment and the suppression. This signaling in the cancer microenvironment affects the progression of cancer. This cancer-promoting or cancer-eliminating process is managed by macrophages. The increase in pro-inflammatory cytokine genes provides suppression of tumor cells in the environment. It was aimed to investigate the effect of Odoroside A on macrophage transformation. Materials and methods: The non-lethal dose of Odoroside A in macrophage cells was determined by CCK-8 analysis. Then, gene expression analysis of cytokines genes and surface markers in RAW264.7 cells was performed by using qRT-PCR. The protein levels of the cytokines that were significant in the gene expression analysis were examined using the ELISA method. Results: Odoroside A significantly increased the expression of IL1 and IL6 genes in RAW264.7 cells, while it significantly decreased the expression of IL10 gene. There was no significant change in IL4 and TGFβ gene expressions. IL1 and IL6 gene expressions, which had a statistically significant increase, were also found to increase significantly at the protein level. Conclusions: When all the data are evaluated together, it has been observed that Odoroside A increases the pro-inflammatory response in RAW264.7 cells, thus providing M1 type macrophage transformation. This suggests that Odoroside A may be effective in tumor elimination of the macrophage-mediated environment in the tumor niche.
Keywords : Odoroside A, makrofaj, sitokinler