Morphological and Anatomical Investigations on in Vitro Micrografts of OHxF 333 / Pyrus elaeagrifolia Interstock / Rootstock Combination in Pears

Authors : Hatice DUMANOĞLU, Aysun ÇELİK, Nurhan BÜYÜKKARTAL, Saeed DOUSTİ

Pages : 269-279

Doi:10.15832/tbd.65549

View : 24 | Download : 5

Publication Date : 2014-08-14

Article Type : Research Paper

Abstract :In this study, the possibility of creation of a specific pear rootstock combination by micrografting was investigated. Two genotypes; ‘Old Home x Farmingdale 333’ insert ignore into journalissuearticles values( Pyrus communis L.); which is a clonal semi dwarf pear rootstock resistant to fireblight and pear decline, and Pyrus elaeagrifolia known as tolerant to Fe-chlorosis, salinity and drought stresses were used to obtain a clonal, semi dwarf and tolerant to the stresses pear rootstock combination. In vitro P. elaeagrifolia seedlings were used as rootstock, and in vitro shoots of ‘OH x F 333’ were used as semi dwarf interstock. Cleft grafting was applied in micrografts. Grafted seedlings were cultured on Murashige and Skoog basal medium with ½ strength of macronutrients for 8 weeks. Cultures were incubated under white fluorescent light, 16 h per day. However, complete darkness was applied for 1 or 2 weeks at the beginning of incubation for the darkness treatments. Graft take success in the control treatment was significantly higher insert ignore into journalissuearticles values(97.9%); than darkness treatments insert ignore into journalissuearticles values(90.5% and 82.5%, respectively);. Ultrastructural observations with transmission electron microscope revealed that dividing cambial initials reached to 2-3 layers and new xylem and phloem elements distinctly differentiated in transversal sections of the graft union after 8 weeks of micrografts in the control and dark treatments. The results indicated that a successful graft union formation occurred. To the best of our knowledge, this is the first report of in vitro micrografting on Pyrus elaeagrifolia Pallas.
Keywords : Micrografting, Graft union, Interstock, Dark treatment