Jasmonic Acid Induced Systemic Resistance in Infected Cucumber by Pythium aphanidermatum

Authors : Ebrahim SABBAGH, Seyed Kazem SABBAGH, Naser PANJEHKEH, Hamid Reza BOLOK-YAZDI

Pages : 143-152

Doi:10.15832/ankutbd.446416

View : 21 | Download : 8

Publication Date : 2018-03-31

Article Type : Research Paper

Abstract :Damping-off disease of cucumber is one of the most destructive diseases of cucumber in worldwide. In this work, the potential of jasmonic acid insert ignore into journalissuearticles values(JA); for induce resistant against damping off disease was investigated. The effect of JA on activity of Polyphenol oxidase insert ignore into journalissuearticles values(PPO);, Peroxidase insert ignore into journalissuearticles values(PO); and Catalase insert ignore into journalissuearticles values(CAT); enzymes and total phenol was assayed by spectrophotometric method. Expression level of three plant defense genes as Lipoxygenase, Cupi4 and Phenylalanine ammonia-lyase genes was analyzed using qRT-PCR method. Drop-plate method was used to assay inhibitory effect of JA on radial growth of fungi. Exogenic application of JA decreased disease severity in the infected plants but did not inhibit mycelia growth on solid medium compared to control. Our results showed that JA application substantially increased the activity of oxidative enzymes at different concentration. The highest enzyme activity was recorded after 48 hours post infection insert ignore into journalissuearticles values(hpi); at a concentration of 400 mg L-1 of JA. Gene expression analysis revealed that JA is differentially able to increase the mRNA transcripts of all tested genes at 48 hpi. The expression level of Cupi4 gene was higher than the other genes in treated plants. Induced systemic resistance by JA was mediated through an enhanced expression of ISR marker genes and increase of antioxidant enzymes activity. Based on these results, we suggest that exogenic application of JA could be considered as plant resistance inducer. 
Keywords : Real time PCR, Defensive enzymes, Elicitors, Damping off, Gene expression, Cpui4 gene