Effects of Cultural Conditions on Exopolysaccharide Production by Bacillus sp. ZBP4

Authors : Erdi ERGENE, Ayşe AVCI

Pages : 386-393

Doi:10.15832/ankutbd.456666

View : 18 | Download : 10

Publication Date : 2018-09-05

Article Type : Research Paper

Abstract :Microbial exopolysaccharides insert ignore into journalissuearticles values(EPSs); are of great interest for the application in various industries due to their gelling, stabilizing, emulsifying, and antioxidant properties. In the present study, EPS production of 12 Bacillus strains were investigated and the best producer, namely Bacillus sp. ZBP4, was selected for further studies in order to determine the effects of fermentation conditions on the biosynthesis of EPSs. Beet molasses was used as substrate in the experiments. The highest amount of EPS was obtained at 60 g L-1 molasses concentration within 24 h. Optimum temperature and pH were determined as 45 oC and 5.0, respectively. Various carbon sources insert ignore into journalissuearticles values(glucose, starch, lactose, whey, mannitol, sucrose, beet molasses); have been tested for EPS production and beet molasses was found as the best. Using inorganic nitrogen source insert ignore into journalissuearticles values(ammonium sulfate); caused a decrease in the production of EPS. Tryptone gave the highest EPS yields amongst the organic nitrogen sources insert ignore into journalissuearticles values(yeast extract, peptone, tryptone); tested. Considerable increase in EPS production insert ignore into journalissuearticles values(1071 mg L-1); has been observed when the experiment was conducted under the optimized conditions insert ignore into journalissuearticles values(using tryptone and 60 g L-1 molasses at pH 5.0 and 45 °C in 24 h); which was 143 mg L-1 before the optimization studies. 
Keywords : Bacillus, Exopolysaccharide EPS, , Beet molasses