In Vitro Indirect Somatic Embryogenesis and Secondary Metabolites Production in the Saffron: Emphasis on Ultrasound and Plant Growth Regulators

Authors : Behnam FİROOZİ, Nasser ZARE, Omid SOFALİAN, Parisa SHEİKHZADE-MOSADEGH

Pages : 1-10

Doi:10.15832/ankutbd.538973

View : 14 | Download : 6

Publication Date : 2019-03-13

Article Type : Research Paper

Abstract :In the present study, the effects of ultrasound and plant growth regulators on in vitro callogenesis and secondary metabolites production in saffron calli were investigated. Accordingly, the saffron corms surface sterilized, sonicated and cultured on different concentrations of plant growth regulators insert ignore into journalissuearticles values(0.5, 1, 2 and 4 mg L-1 2,4-D or NAA in combination with 0.5 and 1 mg L-1 Kin or BAP);. The percentage of callus induction, callus yield insert ignore into journalissuearticles values(fresh weight); and embryogenic callus formation were recorded and secondary metabolites of calli were measured by UV/VIS spectrophotometer three months after culture. The results indicated that sonication of the saffron corm explants significantly increased the in vitro callus induction and growth. So, the highest callus induction insert ignore into journalissuearticles values(100%); and yield insert ignore into journalissuearticles values(4.68 g); was achieved with sonicated explants cultured on MS medium supplemented with 2 mg L-1 1-naphthaleneacetic acid insert ignore into journalissuearticles values(NAA); and 0.5 mg L-1 kinetin insert ignore into journalissuearticles values(Kin);. Somatic embryogenesis was significantly influenced by plant growth regulator regimes and the MS medium supplemented with 0.5 mg L-1 2,4-dichlorophenoxyacetic acid insert ignore into journalissuearticles values(2,4-D); plus 0.5 mg L-1 6-benzylaminopurine insert ignore into journalissuearticles values(BAP); and 0.5 mg L-1 NAA plus 0.5 mg L-1 Kin exhibited the highest percentage insert ignore into journalissuearticles values(75 and 72, respectively); of somatic embryogenesis. Secondary metabolite content of the callus cells was significantly different among the plant growth regulator regimes and the highest production of picrocrocin and safranal were occurred on the medium containing 0.5 mg L-1 2,4-D plus 0.5 mg L-1 BAP and 1 mg L-1 NAA plus 1 mg L-1 BAP.
Keywords : Crocus sativus L, Saffron Secondary Metabolites, Sonication, Tissue culture