Activity Guided Isolation of Nematicidal Constituents from the Roots of Berberis brevissima Jafri and Berberis parkeriana Schneid

Authors : Saqib Ali, İshrat NAZ, Muhammad ALAMZEB, Mamoon URRASHİD

Pages : 108-114

Doi:10.15832/ankutbd.539012

View : 14 | Download : 9

Publication Date : 2019-03-13

Article Type : Research Paper

Abstract :Biological screening of different parts of the selected Berberis species insert ignore into journalissuearticles values(B. brevissima Jafri and B. parkeriana Schneid); showed that methanolic root extract possessed significant efficacy against Meloidogyne javanica insert ignore into journalissuearticles values(a root knot nematode);. From root methanolic extracts of selected Berberis species four isoquinoline alkaloids; jatrorrhizine, dehydrocheilnthifoline, berberine and berberrubine were isolated. Structures of the isolated compounds were determined by using EIMS, 1H and 13C NMR, and other 2D spectroscopic techniques. Percentage juveniles mortality of M. javanica was determined at various concentrations insert ignore into journalissuearticles values(100, 200 and 300 µg mL-1); using carbofuran as control. Berberine possessed the highest nematicidal activity insert ignore into journalissuearticles values(71.33%); followed by jatrorrhizine insert ignore into journalissuearticles values(59.50%);. The in vitro results suggested that these compounds from Berberis species could be potential novel nematicides against M. javanica.
Keywords : Nematicidal activity, Berberis brevissima, Berberis parkeriana, Meloidogyne javanica, isoquinoline alkaloids