- Tarım Bilimleri Dergisi
- Volume:28 Issue:2
- Identification of S-Allele Based Self-incompatibility of Turkish Pear Gene Resources
Identification of S-Allele Based Self-incompatibility of Turkish Pear Gene Resources
Authors : Merve Dilek KARATAŞ, Nahid HAZRATİ, Ezgi OĞUZ, Canan YÜKSEL, Serdar ALTINTAŞ, Mehmet Emin AKÇAY, Ali ERGÜL
Pages : 206-216
Doi:10.15832/ankutbd.823872
View : 30 | Download : 9
Publication Date : 2022-04-25
Article Type : Research Paper
Abstract :Self-incompatibility is considered to be a growth-limiting factor in fruit plants. In species with hermaphrodite flowers, S-locus insert ignore into journalissuearticles values(S-allele); has been accepted to control self-incompatibility, and the genetic control of this locus is provided by multiple genes insert ignore into journalissuearticles values(alleles);. Pear insert ignore into journalissuearticles values(Pyrus communis L.); belongs to the Pomoideae from the Rosaceae family and is found to have great genetic potential in terms of ecological features in Turkey. To protect these cultivation features, national garden collections have been established across the country and Atatürk Horticultural Central Research Institute–Yalova collection is considered as genes bank. Identification of the different features of this collection insert ignore into journalissuearticles values(fruit quality, stress tolerance, self-incompatibility, grafting incompatibility, etc.); is of great importance for its utilization in pear breeding and cultivation. However, to our knowledge, this collection has not been characterized for self-incompatibility trait. In the current study, PCR insert ignore into journalissuearticles values(Polymerase Chain Reaction);-based amplification of the S-allele regions insert ignore into journalissuearticles values(S1, S6, S7, S8); causing the self-incompatibility in 180 pear genotypes obtained from the national pear germplasm was investigated by molecular biological methods based on the comparison of amplified products. In 180 pear genotypes, the S6 allele was the most prevalent one with 63% frequency, while the S8 allele was the least common allele with a rate of 4%. In allele combinations, the S1-S6 allele combination was the most common allele combination with a rate of 18%, and trilateral allele combinations insert ignore into journalissuearticles values(S1-S6-S7 and S1-S6-S8); were observed at a rate of 1%. Findings of the current research will enable the classification of the materials and the analysed material is likely to be used in breeding studies as well as pear cultivation.Keywords : Pear genotypes, self incompatibility, S allel, PCR amplification