Genoprotective Role of Purslane Methanol Extract Against Somatic Mutations Induced by Bifenthrin, a Third Generation Prethyroid Insecticide

Authors : Halit KIZILET, Handan UYSAL

Pages : 583-591

Doi:10.15832/ankutbd.883842

View : 19 | Download : 9

Publication Date : 2022-10-17

Article Type : Research Paper

Abstract :In this study, in vitro and in vivo genotoxic effects of Bifenthrin insert ignore into journalissuearticles values(BIF);, an important insecticide used in agricultural production, storage and processing, were investigated. The genoprotective properties of purslane insert ignore into journalissuearticles values(Portulaca oleracea L.); against the genotoxic effects of BIF were also determined by using the methanol insert ignore into journalissuearticles values(POmet); extract of this plant. In vivo experiments were performed with somatic mutation and recombination test insert ignore into journalissuearticles values(SMART); in Drosophila melanogaster. In in vitro studies, human peripheral blood cultures were prepared and different concentrations of BIF were applied to lymphocyte cells in accordance with the procedure of both the micronucleus insert ignore into journalissuearticles values(MN); and sister chromatid exchange insert ignore into journalissuearticles values(SCE); assay. The results obtained from all applied tests showed that BIF is genotoxic and induces chromosomal mutations. Later, another experiment was conducted and it was determined that the genotoxic effects of BIF were reduced with POmet insert ignore into journalissuearticles values(1:1 v/v);. This result, which was observed in all in vivo and in vitro tests, shows that purslane plant is a potent radical scavenger. Due to the healing properties of POmet, gas chromatography-mass spectrometry insert ignore into journalissuearticles values(GS-MS); method was used to determine the components in its content. Some of the components found in the highest ratio in this extract are γ-sitosterol insert ignore into journalissuearticles values(21.86%);, 13-docosenamide, insert ignore into journalissuearticles values(13.30%);, palmitic acid insert ignore into journalissuearticles values(12.85%);, stigmasteol insert ignore into journalissuearticles values(6.64%);, campesterol insert ignore into journalissuearticles values(5.69%);, linoleicacid insert ignore into journalissuearticles values(5.46%); and 2-methyl-1-hexadecanol insert ignore into journalissuearticles values(3.88%);.
Keywords : Drosophila melanogaster, Portulaca oleracea, Household insecticides, Human peripheral blood cultures, Gas chromotography mass spectrometry