Effects of Plant Growth Regulators on the Usage of In Vitro Stem Disc Culture for Mass Seedling Production in Kahramanmaraş Garlic (Allium sativum L.)

Authors : Ayşe GÜLÇEBİ, İrfan Ersin AKINCI

Pages : 550-560

Doi:10.33462/jotaf.1117827

View : 111 | Download : 84

Publication Date : 2023-09-26

Article Type : Research Paper

Abstract :Anatolia is one of the important production areas of garlic. Garlic production is conducted by using the head or the cloves inside the heads, which are actually the consumed part of the garlic, as propagation material. However, due to the use of cloves, which are the most valuable part of the market, as reproduction material, the profit is reduced by about 10%. The study aims to provide an alternative propagation material to reduce the losses resulting from this practice in local Kahramanmaraş garlic production. For this purpose, generating plantlets directly from stem discs of the garlic cloves in vitro and the effects of different growth regulators have been studied. For this, the plant growth regulators added to MS media as BAP insert ignore into journalissuearticles values(1.0, 1.5 and 2.0 mg l-1);, GA3 insert ignore into journalissuearticles values(0.5, 1.0 and 1.5 mg l-1);, 2-IP insert ignore into journalissuearticles values(0.75, 1.00 and 1.25 mg l-1);; kinetin insert ignore into journalissuearticles values(1.0, 2.0 and 3.0 mg l-1); and TDZ insert ignore into journalissuearticles values(0.75, 1.00 and 1.25 mg l-1); were tested. In the study number of explants, number of infected explants, number of healthy explants, number of developed explants, healthy explant rate, developed explant rate, number of callused explants, callus growth rate, number of proliferated explants, proliferation rate, proliferation number, number of rooted explants, rooting rate and number of roots were investigated. However, shoot ratios, shoot numbers, and callus formation were the main focus. The highest rates of proliferation were found in 2-IP insert ignore into journalissuearticles values(53.8%, 45.5%, and 40.0% at 1.00, 0.75, and 1.25 mg l-1 dosages, respectively); and Kinetin insert ignore into journalissuearticles values(35.3% at 2.00 mg l-1);. The maximum shoot number was reached with 2-IP at the dose of 1.00 mg l-1 as 1.9 shoot/explants. Kinetin at 3.00 mg l-1 and 2IP at 1.25 mg l-1 were the other successful applications with 1.8 shoots. This study indicated promising results to obtain plantlets directly from the clove’s stem discs and including them into seedling production for the mass production of garlic.
Keywords : BAP, GA3, 2 IP, Kinetin, TDZ, Sarımsak, Gövde disk kültürü