Determination of Nuclear DNA Content and Chromosome Number of Verbascum scamandri Murb. (Scrophulariaceae)

Authors : Gülru YÜCEL, Ebru CAMBAZ, Nurşen ÇÖRDÜK

Pages : 397-406

Doi:10.7161/omuanajas.1286961

View : 18 | Download : 27

Publication Date : 2023-07-04

Article Type : Research Paper

Abstract :The study aimed to determine the chromosome number and nuclear DNA content of an endemic plant, Verbascum scamandri, using 14-week-old plants germinated from seed and to determine the genetic stability based on flow cytometry analyses in callus tissue induced MS medium containing 1 mg/L Kin + 1 mg/L 2,4-D and 9-week-old propagated plants, induced and developed on MS medium containing 2 mg/L Kin + 0.1 mg/L 2,4-D. In the mitotic chromosome counts showed that V. scamandri had 2n = 32 chromosome number. Flow cytometric analysis revealed that 14-week-old in vitro grown plants have 0.73 pg/2C ± 0.01, callus tissue has 0.76 pg/2C ± 0.02 and propagated plantlets have 0.79 pg/2C ± 0.01. The results proved that propagated plants had similar DNA content to the seed-derived plants which showed analysed plants were genetically stable.
Keywords : Çekirdek DNA İçeriği, Kromozom, Doku Kültürü, Sığırkuyruğu