Determination of HIF1Ars11549465 Polymorphism in Elite Skiers

Authors : Ömer KAYNAR, Fatih BİLİCİ, Korkut ULUCAN, Canan SERCAN

Pages : 328-331

Doi:10.15314/tsed.544566

View : 15 | Download : 10

Publication Date : 2019-09-15

Article Type : Research Paper

Abstract :ABSTRACT Studies in sport genetics have increased nowadays and the identification of genotype and allelic distributions of candidate genes in different athletes has provided important information to sports scientists. One of the candidate genes studied in sports genetics is the hypoxia-inducible factor 1-alpha insert ignore into journalissuearticles values(HIF1A); gene, which is associated with the endurance phenotype. In our study, we aimed to determine the genotypic and allelic distribution of HIF1A rs11549465 polymorphism in Turkish Elite Ski Runners. 34 insert ignore into journalissuearticles values(23 male, 11 female); athletes were included in the study, and 1 cc of blood samples taken for routine analysis after the approval of the ethics committee was used for DNA isolation. The commercial kit was used for DNA isolation and genotyping was performed by Real-time PCR method. The number and percentage of CC, CT and TT genotypes of the HIF1A rs11549465 polymorphism were 24 insert ignore into journalissuearticles values(72.72%);, 8 insert ignore into journalissuearticles values(24.24%); and 1 insert ignore into journalissuearticles values(3.03);, respectively. In male skiers CC, CT and TT genotype numbers and percentages were determined as 17 insert ignore into journalissuearticles values(55.51%);, 4 insert ignore into journalissuearticles values(12.12%); and 1 insert ignore into journalissuearticles values(3.03%); respectively. In female, CC and TT genotypes were determined as 7 insert ignore into journalissuearticles values(21.21%); and 4 insert ignore into journalissuearticles values(12.12%);. We detected no TT genotype in female athletes. When we look at allel numbers, C allele was determined as 56 insert ignore into journalissuearticles values(84.84%); and T allele was determined as 10 insert ignore into journalissuearticles values(15.15%);. This is the first study on HIF1A rs11549465 polymorphism on Turkish elite ski runners. The higher prevalence of CC genotype and C allele in the athlete group is similar to the previous studies on different groups of athletes. We believe that this study will be an important reference for further studies.
Keywords : Sport genetics, cross country, athletic performance