Immunological Diagnostic of Phytophthora infestans from Host Tissues (Potato) by ELISA Method

Authors : Touseef HUSSAIN, Bir Pal SINGH, Firoz ANWAR

Pages : 385-392

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Publication Date : 2017-10-26

Article Type : Research Paper

Abstract :Oomycete pathogens of the genus Phytophthora are the most destructive plant pathogens known. They spread mainly through the movement of infested soil, water and infected plants and plant material. Especially damaging as a source of inoculum are those plants/seeds that are infected but do not show signs of symptoms either because the disease has not yet progressed to the stage where symptoms are evident, or due to suppression of symptom development by the use of fungicides. In our indirect ELISA method, P.infestans exhibited strong positive reaction with sporangia insert ignore into journalissuearticles values(2.256);, mycelium insert ignore into journalissuearticles values(1.256); as well as oospores insert ignore into journalissuearticles values(2.286); whereas no reaction with other fungal pathogens of potato. P.infestans was detected by indirect ELISA in potato leaves insert ignore into journalissuearticles values(1.212); and tubers insert ignore into journalissuearticles values(1.201);. Our study was to confirm the detection of P.infestans irrespective of inoculum present in the host tissues insert ignore into journalissuearticles values(0.435);, by which planting material by quarantine dept., horticultural dept. from one state to another. It could be used as reliable routine diagnostic test to replace current identification and isolation, detection methods .
Keywords : Phytophthora infestans, oospores, potato, ELISA