Detection of Cucumber Mosaic Virus (CMV) in Cucurbits by TaqMan Real-Time RT-PCR in Hakkari Province, Türkiye

Authors : Ayşenur Biltekin, Nevin Akdura

Pages : 825-834

Doi:10.30910/turkjans.1466706

View : 63 | Download : 56

Publication Date : 2024-07-24

Article Type : Research Paper

Abstract :Cucumber mosaic virus (CMV) is among the important viruses that can infect many crops. In 2022, this study was conducted in the Kırıkdağ, Otluca, and Merzan areas of the Hakkari province of Türkiye to investigate the presence of CMV in cucurbits exhibiting viral disease symptoms (yellowing, necrosis, mosaic, and deformity). A total of 50 samples; 7 watermelons (Citrullus lanatus), 3 melons (Cucumis melo L.), 17 cucumbers (Cucumis sativus L.), 8 zucchinis (Cucurbita pepo L.), 9 pumpkins (Cucurbita moschata), 2 gourds (Lagenaria siceraria) and 4 gherkins (Cucumis melo var. flexuous) were collected. The presence of CMV in the samples was investigated by TaqMan real-time RT-PCR using probes and primers specific to the 105 bp region of partial the coat protein gene of the virus. CMV-specific viral RNA was detected in 6 (12%) of the 50 samples analyzed. CMV was detected most commonly in the Otluca area and melon (33.3%) among cucurbit varieties. CMV agent was not detected in the samples collected from the Merzan area. The TaqMan real-time RT-PCR assay used in this study demonstrated that the method can be widely used for the general detection of CMV in a wide range of hosts. This is the first report on the detection of CMV in cucurbits in the Hakkari province of Türkiye.
Keywords : Hıyar mozaik virüsü, kabakgiller, TaqMan real time RT PCR, Hakkari