Histochemical and ultrastructural analysis of macromolecules in trophocytes of the Oriental cockroach, Blatta orientalis (L., 1758) (Blattodea: Blattidae)

Authors : Tuğba ZÜLFİKAROĞLU, Gamze TURGAY İZZETOĞLU, Mehmet Salih YIKILMAZ, Savaş İZZETOĞLU

Pages : 87-100

Doi:10.16970/entoted.1118639

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Publication Date : 2023-04-25

Article Type : Research Paper

Abstract :The fat body is a major storage area for glycogen, lipid and protein. The trophocyte is the main cell of fat body and stores these macromolecules. The fat body consists of two parts; peripheral and perivisceral. Peripheral fat body is located below the integument while perivisceral fat body is around the digestive tract. The study was conducted in EGEMIKAL Analysis Laboratory and Histology Laboratory of Ege University between 2018 and 2020. The fat body contents of insects at all stages were examined comparatively in three selected sections through histochemical and ultrastructural studies. We identified macromolecules stored in the trophocytes. Both the granular form of proteins and asterisk structure of glycogen localized around the lipid droplets were observed clearly. It was found that accumulation of protein continued in the trophocytes, but glycogen accumulation decreased considerably in adults compared to all nymphal stages. We also found that larger lipid droplets were stored in the PF fat body, while glycogen and protein accumulation was much higher in the PV fat body. These results may contribute to understanding of the mechanisms underlying activities such as amino acid, nitrogen, lipid and carbohydrate metabolism and protein synthesis in insects.
Keywords : Blatta orientalis, böcek yağ dokusu, histokimya, TEM, trofosit