Identification of odorant receptor protein genes in two Cephid stem borers (Hymenoptera: Cephidae) by high-throughput sequencing

Authors : Mahir BUDAK

Pages : 307-316

Doi:10.16970/entoted.1337346

View : 58 | Download : 56

Publication Date : 2023-10-09

Article Type : Research Paper

Abstract :Insects are well adapted organisms to the terrestrial life on Earth. The evolution of the odorant receptor family is one of the causes underpinning this remarkable adaptation. Odorant receptors insert ignore into journalissuearticles values(ORs); sense aromas in the environment and cause the insect to respond. The ability of phytophagous insects to detect odor signals from their hosts is crucial for mating, oviposition, and feeding. The family of odorant receptor genes in Cephidae, pest on some economically important plants, is little understood. Bioinformatic tools were used to analyze the genomic data of the two pest species, Syrista parreyssii insert ignore into journalissuearticles values(Spinola, 1843); insert ignore into journalissuearticles values(Hymenoptera: Cephidae); insert ignore into journalissuearticles values(a rose pest); and Pachycephus smyrnensis J.P.E.F. Stein, 1876 insert ignore into journalissuearticles values(Hymenoptera: Cephidae);, insert ignore into journalissuearticles values(a poppy pest);, to determine their odorant receptors. The whole genome sequencing of P. smyrnensis collected in Sivas in 2020 was performed by next generation sequencing and short reads of S. parreyssii genome were obtained from previous studies. Following bioinformatic analyses, 67 and 82 putative odorant receptor genes were identified and annotated for P. smyrnensis and S. parreyssii, respectively. The ORs of these two species were found to be organized as repetitive genes in five separate clusters. No species-specific OR genes were identified in any of the investigated species. As a result, it was hypothesized that host specificity was acquired through the combined effect of multiple ORs.
Keywords : Cephini, yeni nesil dizileme, koku almaçları, tekrarlayan genler, Testereli arılar