A Phylogenetic and Genotyping Study of Bovine Herpesvirus Type 4 (BHV-4) in Turkey

Authors : Bahattin Taylan KOÇ

Pages : 61-66

Doi:10.30782/jrvm.641125

View : 13 | Download : 7

Publication Date : 2020-07-29

Article Type : Research Paper

Abstract :Infertility and reproductive infections are the huge problems for the diary management throughout the world. Bovine herpesviruses act an enormous role in these complicated problems. Bovine Herpesvirus Type 1 insert ignore into journalissuearticles values(BHV-1); is the most outstanding herpesvirus causing genital and uterine tracts infections among other reproductive viral agents, however, Bovine Herpesvirus Type 4 insert ignore into journalissuearticles values(BHV-4); is also responsible in terms of similar symptoms and diseases. The main aims of the study are both to investigate the underlying potential presence of BHV-4 in subclinical uterine tract infection, and both to perform molecular and recombination analyses. A herd including 25 repeat breeder cows were investigated by BHV-4. Two out of them were determined BHV-4 infected after a series of Polymerase Chain Reactions insert ignore into journalissuearticles values(PCRs); tests which able to amplify partial Glycoprotein B insert ignore into journalissuearticles values(gB); and Thymidine Kinase insert ignore into journalissuearticles values(TK); gene regions. Obtained sequences were analyzed by using phylogenetic and recombinational software, and two Maximum Likelihood insert ignore into journalissuearticles values(ML); tree have been constructed. To results, novel Turkish BHV-4 sequences fell into Genotype I in both constructed Maximum Likelihood insert ignore into journalissuearticles values(ML); phylogenetic trees, however, no recombination evidence has been observed in relevant software. This report is the first genotyping study of BHV-4 from Turkey. This study showed that Turkish BHV-4 substantially originated from European strains and might be observed in different clinical presentations. This suggests that BHV-4 should be deeply investigated by further molecular techniques and included in diagnostic panels for reproductive diseases viruses.
Keywords : Reproductive Infection, Bovine herpesvirus Type 4 BHV 4, , Repeat breeder, Infertility, Genotyping, Turkey