Investigation of Energy Metabolism in The Different Tissues of Colon Cancer Xenograft Models Based on The TCA and Cori Cycles and Glycolysis

Authors : Esra BULUT ATALAY, Hülya AYAR KAYALI

Pages : 117-124

Doi:10.29137/umagd.1142303

View : 10 | Download : 5

Publication Date : 2023-01-31

Article Type : Research Paper

Abstract :The metabolism is reprogrammed in cancer cells to meet the requirement of their malignant properties. Some specific metabolites can contribute to the malignant transformation in different cancer types. This study aimed to determine the level of glucose, pyruvate, lactate, citrate, alpha-ketoglutarate insert ignore into journalissuearticles values(α-KG);, 2-hydroxyglutarate insert ignore into journalissuearticles values(2-HG);, succinate, fumarate, malate, and oxaloacetate in the different tissues insert ignore into journalissuearticles values(tumor, colon, brain, and liver); of xenograft models generated by a colon adenocarcinoma cell line insert ignore into journalissuearticles values(SW480);. Glucose and pyruvate levels were determined by the 3,5-dinitrosalicylic acid insert ignore into journalissuearticles values(DNS); method and pyruvate assay, respectively. The level of other metabolites was determined by the HPLC analysis. The results show that D-2-HG is highly produced in the tumor tissues insert ignore into journalissuearticles values(120.2 mg/L);, but it could not be detected in liver tissue. In conclusion, TCA and Cori cycles and Glycolysis were investigated in the different tissues of colon cancer xenograft models and found that some metabolites produced tissue-specific.
Keywords : Kolon kanseri, ksenograft model, hedeflenmiş metabolomiks, metabolit, HPLC