The effect of humic acid on rapeseed (Brassica napus L.) plant growth, heavy metal uptake, phytoremediation parameters (BCF, TF and TI), and antioxidant activity in heavy metal polluted soil

Authors : Sibel BOYSAN CANAL, Mehmet Ali BOZKURT, Hilal YILMAZ

Pages : 237-248

Doi:10.29133/yyutbd.997850

View : 17 | Download : 5

Publication Date : 2022-06-30

Article Type : Research Paper

Abstract :The aim of this study was to investigate the effects of humic acid insert ignore into journalissuearticles values(HA); applications on rapeseed insert ignore into journalissuearticles values(Brassica napus L.); growth, heavy metal uptake, bioconcentration factor insert ignore into journalissuearticles values(BCF);, translocation factor insert ignore into journalissuearticles values(TF);, tolerance index insert ignore into journalissuearticles values(TI);, catalase insert ignore into journalissuearticles values(CAT);, ascorbate peroxidase insert ignore into journalissuearticles values(APX); enzyme activities and hydrogen peroxide insert ignore into journalissuearticles values(H2O2); content in polluted soil with lead insert ignore into journalissuearticles values(Pb);, chromium insert ignore into journalissuearticles values(Cr);, cadmium insert ignore into journalissuearticles values(Cd);, and zinc insert ignore into journalissuearticles values(Zn);. Three doses of HA insert ignore into journalissuearticles values(Control, HA1:500 mg kg-1, HA2:1000 mg kg-1, HA3:2000 mg kg-1); were applied in pots. HA1, HA2, and HA3 applications increased plant growth parameters compared to polluted soil. Compared to the control, HA applications in polluted soil increased the Pb, Cr, Cd, and Zn concentrations in the plant. However, HA applications in polluted soil significantly decreased the heavy metal content in roots and shoots of the plant compared to polluted soil. BCF in both roots and shoots of the plants were greater than 1 for Pb, Cr, Cd, and Zn. However, specifically HA2 application decreased the shoot and root BCF values in polluted soil. TF was smaller than 1 in Pb, Cr, Cd, and Zn in polluted soil. On the other hand, HA applications for Cd increased TF values. Shoot TI decreased 17.37 %, and root TI decreased 9.09% in polluted soil. CAT and APX enzyme activities and H2O2 increased significantly in polluted soil. However, HA applications decreased CAT and APX enzyme activities and H2O2 content in rapeseed. It is concluded that HA application in Pb, Cr, Cd, and Zn polluted soil has a remedial effect on the development of rapeseed by reducing heavy metal content and oxidative stress.
Keywords : Antioxidative activity, Heavy Metal, Humic Acid, Plant Growth, Rapeseed Brassica napus L,