Novel g.2055A>C and g.2064T>A Polymorphisms of KISS1 Gene and Its Association with Reproductive Traits in Local Indonesian Goats

Authors : Achiriah FEBRİANA, Edy KURNİANTO, Abu BAKAR, Sabir SABİR, Yoyok Budi PRAMONO, Sutopo SUTOPO

Pages : 766-774

Doi:10.29133/yyutbd.1136214

View : 12 | Download : 4

Publication Date : 2022-12-30

Article Type : Research Paper

Abstract :Kisspeptins are known as neuropeptides encoded by the KISS1 gene, which potentiates GnRH neuron excitability. Unfortunately, the role of the KISS1 gene in reproductive traits remains unclear in Indonesian native goat breeds. The current study purposed to detect the genetic variation of the KISS1 gene and investigate the association with reproductive traits in goats to acquire marker-assisted selection insert ignore into journalissuearticles values(MAS); for the breeding program. Further, ninety blood samples from randomly selected animals were used for DNA isolation and SNP genotyping. The blood serum was collected from sixteen treated goat does to assess FSH levels using ELISA method. The differences between studied parameters were determined using independent samples T-test from SAS Software. Two SNPs, g.2055A>C insert ignore into journalissuearticles values(SNP1); and g.2064T>A insert ignore into journalissuearticles values(SNP2); were discovered in intron 1 of KISS1 gene in Indonesian native goat breeds. Two genotypes were identified from each SNPs: AA and AC at SNP1 and TT and TA at SNP2. The allele A insert ignore into journalissuearticles values(0.96); at SNP1 and allele T insert ignore into journalissuearticles values(0.93); at SNP2 were discovered as dominant alleles in the population. Furthermore, chi-square test showed that both SNPs were under Hardy-Weinberg equilibrium. The variants of KISS1 gene have no effect on litter size insert ignore into journalissuearticles values(p>0.05);. Moreover, AA genotype of SNP1 had a higher FSH level than that observed in AC genotype insert ignore into journalissuearticles values(p<0.0001);, especially in the follicular phase. SNP2 does not correlate with FSH level relatively insert ignore into journalissuearticles values(p=0.23);. Furthermore, using of AA genotype at SNP1 of KISS1 gene as MAS for the breeding selection program could escalate the reproductive traits in goats
Keywords : FSH, KISS1 gene, Litter size, SNP