EVALUATION OF THE POTENTIAL ANTIBACTERIAL ACTIVITY OF BERBERIS CRATAEGINA DC.: WITH FOCUS ON QUORUM SENSING INHIBITION OF CHROMOBACTERIUM VIOLACEUM ATCC 12472

Authors : Fatma Tuğçe GÜRAĞAÇ DERELİ, Evren ARIN, Ebru ÖNEM

Pages : 178-184

Doi:10.33483/jfpau.1165905

View : 12 | Download : 8

Publication Date : 2023-01-20

Article Type : Research Paper

Abstract :Objective: In the present study described here, we set out to look into the quorum sensing inhibitory activity of the methanolic seed extract of Berberis crataegina DC. through quorum sensing -controlled inhibition of violacein pigment production in Chromobacterium violaceum ATCC 12472. In addition, the antibacterial activity of the extract on various Gram-negative and Gram-positive standard strains was evaluated. Also phenolic contents in the extract were detected by using HPLC analysis. Material and Method: “The phytochemical profile of the seed extract was performed by High-Performance Liquid Chromatography technique. Antibacterial activity assays were performed on the extract using the agar well method and inhibition of the violacein pigment production was investigated spectrophotometrically. Result and Discussion: According to antibacterial activity results Gram negative bacteria were more resistant than Gram positive bacteria and violacein pigment production was inhibited by 66% percentage. Phytochemical analysis results also showed that the major component in the extract is chlorogenic acid, with a value of 1974.91 µg/mL.
Keywords : Antibacterial, HPLC, phenolic content, quorum sensing