CURCUMIN, THE BIOACTIVE COMPOUND OF TURMERIC, MAY IMPROVE THE ANTI-MALIGNANT PROPERTY OF GEMCITABINE IN PROSTATE CANCER CELLS

Authors : Yalçın ERZURUMLU, Hatice Kübra DOĞAN, Deniz ÇATAKLI

Pages : 739-751

Doi:10.33483/jfpau.1232868

View : 73 | Download : 122

Publication Date : 2023-09-20

Article Type : Research Paper

Abstract :Objective: The aim of this study was to investigate the possible synergistic effect of curcumin on the anticancer features of gemcitabine on prostate cancer cells. Material and Method: The human prostate adenocarcinoma cell line LNCaP was used in the studies. The effect of the co-administration of gemcitabine and curcumin on the viability of LNCaP cells was investigated by the WST-1 assay. Autophagy, ubiquitin-proteasome system insert ignore into journalissuearticles values(UPS);, unfolded protein response insert ignore into journalissuearticles values(UPR); and cell death-associated proteins, androgenic signaling, proto-oncogenic, angiogenic and epithelial-mesenchymal transition insert ignore into journalissuearticles values(EMT); associated protein levels were investigated by immunoblotting studies. Result and Discussion: Our results showed that curcumin potentiated the anticancer effects of gemcitabine on LNCaP cells. Co-administration of curcumin and gemcitabine strengthened the suppressive effect of gemcitabine on cell viability. Moreover, co-administration modulated the autophagy, more strongly stimulated UPS and UPR, suppressed androgenic signaling, led to the activation of cell death-related poly [ADP-ribose] polymerase 1 insert ignore into journalissuearticles values(PARP-1); and caspase-3 and strongly suppressed the expression levels of proto-oncogenic c-Myc and angiogenic vascular endothelial growth factor-A insert ignore into journalissuearticles values(VEGF-A);. In addition, it was determined that co-administration negatively regulated EMT by stimulating E-cadherin expression and suppressing N-cadherin level. These results suggest that the combined usage of gemcitabine and curcumin may offer a potent therapeutic approach for prostate cancer by enhancing the anticancer effects of gemcitabine.
Keywords : Gemsitabin, katlanmamış protein yanıtı, kurkumin, otofaji, prostat kanseri