COENZYME Q0 INHIBITS CELL PROLIFERATION AND MODULATES MAPK AND AKT SIGNALLING PATHWAYS IN HUMAN CHRONIC MYELOID LEUKEMIA K562 CELLS

Authors : Ecem KAYA SEZGİNER, Ali YAPRAK, Arzu Zeynep KARABAY

Pages : 761-769

Doi:10.33483/jfpau.1286359

View : 58 | Download : 74

Publication Date : 2023-09-20

Article Type : Research Paper

Abstract :Objective: This study evaluated the antiproliferative and pro-apoptotic effects of coenzyme Q0 insert ignore into journalissuearticles values(CoQ0); in human chronic myeloid leukemia K562 cell line. Material and Method: The cytotoxic effect of CoQ0 on human chronic myeloid leukemia cell line, K562 was determined by MTT test. The activity of caspase-3, expression of proteins involved in apoptosis, MAPK and AKT signaling pathways were determined with enzymatic assay and western blot analysis, respectively. Result and Discussion: Results showed that CoQ0 inhibited cell viability of K562 cells at 5 μM and higher concentrations and Bax protein expression was significantly decreased at 12.5 μM concentration of CoQ0. However, CoQ0 did not significantly affect caspase 3 activity and Bcl-2 protein expression. p-c-Raf insert ignore into journalissuearticles values(Ser259); protein expression was significantly decreased at 12.5 μM of CoQ0. Treatment with 10 μM of CoQ0 induced significantly phosphorylation of p38 MAPK and 12.5 μM CoQ0 caused a nonsignificant decrease in p-ERK1/2 protein expression in K562 cell line. Interestingly, in K562 cells, phosphorylation of Akt insert ignore into journalissuearticles values(Ser473); was diminished at 12.5 μM of CoQ0, with no change observed in p-Akt insert ignore into journalissuearticles values(Thr308); protein expression among groups. In conclusion, CoQ0 inhibited cell proliferation and suppressed phosphorylation of c-Raf insert ignore into journalissuearticles values(Ser259);, Akt insert ignore into journalissuearticles values(Ser473);, but not ERK1/2 in K562 cells. There is still a need for new insights into the anticancer mechanisms of CoQ0 and develop treatment strategies for chronic myeloid leukemia.
Keywords : Koenzim Q0, kronik miyeloid lösemi, K562