ANTIFUNGAL ACTIVITY OF METHYL GALLATE AND SYRINGIC ACID ISOLATED FROM ASTERISCUS GRAVEOLENS AGAINST FUSARIUM OXYSPORUM F. SP. ALBEDINIS

Authors : Zeyneb Belhi, Zahira Roucham, Noureddine Boulenouar, Abdelkrim Cheriti

Pages : 1303-1312

Doi:10.33483/jfpau.1441741

View : 35 | Download : 27

Publication Date : 2024-11-25

Article Type : Research Paper

Abstract :Objective: The objective of this study was to isolate and identify the compounds responsible for the antifungal activity against Fusarium oxysporum f. sp. albedinis (Foa) from Asteriscus graveolens aerial parts extract, and to evaluate the effects in vitro of selected compounds for control of Fusarium wilt. Material and Method: We reveal the presence of the phenolic compounds in Asteriscus graveolens, from which the antifungal activities of aerial parts extracts were investigated for effects on the growth of mycelia against Fusarium oxysporum f. sp. albedinis (Foa) by direct bioautography. The antifungal compounds were isolated from A. graveolens extract using silica gel column chromatography and thin-layer chromatography. Structural identification of the antifungal compounds was conducted using NMR (1H and 13C) spectrophotometry and LC-MS. Result and Discussion: The isolated compounds were identified as methyl gallate (MG) and syringic acid (SA) based on comparing their spectral and physical data with the literature.
Keywords : Asteriscus graveolents, Fusarium oxysporum f sp Albedinis, metil gallat, siringik asit