VILDAGLIPTIN IMPROVES DETRUSOR CONTRACTILITY IN A MOUSE MODEL OF CYCLOPHOSPHAMIDE-INDUCED OVERACTIVE BLADDER

Authors : Seçkin Engin, Elif Nur Barut, Merve İsmailoğlu Karaca, Melis Nazlı Yanık

Pages : 53-61

Doi:10.33483/jfpau.1507431

View : 92 | Download : 111

Publication Date : 2025-01-20

Article Type : Research Paper

Abstract :Objective: Overactive bladder (OAB) is a common urological disorder associated with detrusor overactivity linked to local tissue inflammation resulting in bladder hypersensitivity. The present study was aimed to investigate the therapeutic potential of vildagliptin (VIL), an anti-diabetic drug with anti-inflammatory effects, in a mouse model of cyclophosphamide (CP)-induced OAB. Material and Method: To induce an animal model of OAB, female Balb/c mice were intraperitoneally (i.p) injected with CP (80 mg/kg) every two days for 7 days. Then, mice were orally treated with saline (OAB model), VIL (10 or 50 mg/kg/day) or solifenacin (10 mg/kg/day) for 7 consecutive days. On the 17th day of experiment, organ-bath experiments were performed using isolated mouse detrusor muscle to evaluate tissue contractility. In another set of mice, bladder inflammation was assessed by Evans blue extravasation. Result and Discussion: Carbachol-induced contraction of detrusor strips significantly increased in OAB mice, which was reversed by treatment with VIL at 50 mg/kg or solifenacin. In addition, VIL treatment (50 mg/kg) reduced relative bladder weight and Evans blue dye extravasation into the bladders in CP-injected mice, demonstrating the inhibitory effect of VIL on CP-induced bladder inflammation. Our results showed that VIL ameliorated detrusor overactivity in a mouse model of CP-induced OAB by partially suppressing bladder inflammation.
Keywords : Aşırı aktif mesane, detrusor, evans mavisi, mesane inflamasyonu, solifenasin, vildagliptin