Characterization of M3 Mutants of Seri 82 Bread Wheat Cultivar Using Functional Markers

Authors : Ziya DUMLUPINAR, İlker YÜCE, Özgür KEKİLLİ, Harun OCAKTAN, Hatice OSANMAZ, Münire TOPSAKAL, Tuğba BAŞKONUŞ, Hülya KİRAZ, Numan Yıldırım GÜROCAK, Fatih Mehmet KILINÇ

Pages : 194-202

View : 16 | Download : 6

Publication Date : 2019-10-01

Article Type : Research Paper

Abstract :In this study, Seri 82 bread wheat and 6 mutant lines insert ignore into journalissuearticles values(Seri 82-M3-1, Seri 82-M3-2, Seri 82- M3-3, Seri 82-M3- 4, Seri 82-M3-5 and Seri 82-M3-6); were screened for some traits using 17 allele specific DNA markers. In the study Seri 82 bread wheat and 6 mutant lines characterized for alleles gluten strength insert ignore into journalissuearticles values(Bx7OE marker);, yellow rust Yr45 insert ignore into journalissuearticles values(Xwgp115 marker); and Yr51 insert ignore into journalissuearticles values(SUN104 marker);, stem rust Sr49 insert ignore into journalissuearticles values(SUN209 and SUN479 markers);, vernalisation Vrn-A1 insert ignore into journalissuearticles values(VRN1 marker);, dwarfing Rht-B1a insert ignore into journalissuearticles values(tall); insert ignore into journalissuearticles values(BF-WR1 marker);, Rht-B1b insert ignore into journalissuearticles values(short); insert ignore into journalissuearticles values(BF-MR1 marker);, Rht-D1a insert ignore into journalissuearticles values(tall); insert ignore into journalissuearticles values(DF2-WR2 marker);, Rht-D1b insert ignore into journalissuearticles values(short); insert ignore into journalissuearticles values(DF MR2 marker);, Rht8 insert ignore into journalissuearticles values(WMS261 marker);, high protein content Gpc-B1 insert ignore into journalissuearticles values(UHW89 marker);, rye translocation insert ignore into journalissuearticles values(RIS, NOR and SCM9 markers);, grain hardness Pina insert ignore into journalissuearticles values(Pina-D1 marker); and waxy Wx-A1 insert ignore into journalissuearticles values(SUN1 marker);. According to the results, 61 polymorphic bands obtained from 17 DNA markers and the average allele number was 3.58. The highest band numbers were obtained from RIS and SCM9 markers with 6 bands, while the lowest band numbers were obtained from Bx7OE, Xwgp115 and SUN209 markers. The average polymorphism information content insert ignore into journalissuearticles values(PIC); value was determined as 0.79, while the highest PIC value was obtained from BF-WR1 markers as 0.99. As the allele specific markers evaluated stem rust Sr49 was determined in Seri 82, Seri 82-M3-2, Seri 82-M3-3, Seri 82-M3-4 and Seri 82-M3-6 genotypes while dwarfing gene insert ignore into journalissuearticles values(short); screened in Seri 82-M3-2 genotype and dwarfing gene insert ignore into journalissuearticles values(tall); determined on the Seri 82, Seri 82-M3-1, Seri 82-M3-3, Seri 82-M3-4, Seri 82-M3-5 and Seri 82-M3-6 genotypes. Rht8 dwarfing gene was found in Seri 82-M3-1, Seri 82-M3-4 and Seri 82-M3-6 genotypes and rye translocation was found in all mutant lines. 
Keywords : Bread wheat, Functional DNA markers, Mutagenesis, Mutant