The Resistance of Some Tomato Lines against Tomato Spotted Wild Virus, Tomato Yellow Leaf Curl Virus and Root Knot Nematodes (Meloidogyne spp.) by Molecular Markers

Authors : Hüseyin BASIM, Osman KANDİL, Ramazan İĞDİRLİ, Mehmet MOR

Pages : 401-405

Doi:10.47115/bsagriculture.1161306

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Publication Date : 2022-10-01

Article Type : Research Paper

Abstract :In this study, it was attempted to determine the resistance of different tomato lines to tomato spotted wilt virus insert ignore into journalissuearticles values(TSWV);, tomato yellow leaf curl virus insert ignore into journalissuearticles values(TYLCV);, and root-origin nematodes insert ignore into journalissuearticles values(Meloidogyne spp.); using molecular DNA markers. For this purpose, out of 96 different tomato lines to be tested, Sw5-2 for resistance to tomato spotted wilt ripening virus insert ignore into journalissuearticles values(TSWV);, Ty3P6-25 for resistance to tomato yellow leaf curl virus insert ignore into journalissuearticles values(TYLCV); and the DNA marker Mi23, which determines resistance to root-knot nematodes insert ignore into journalissuearticles values(Meloidogyne spp.);, were used by PCR. In this study, Ty3P6-25, the marker that determines resistance to TYLCV, was found to be susceptible insert ignore into journalissuearticles values(rr); in 34 tomato lines, heterozygous resistant insert ignore into journalissuearticles values(Rr); in 56 tomato lines, and homozygous resistant insert ignore into journalissuearticles values(RR); in 4 tomato lines. In addition, no results were obtained in 2 tomato lines. Marker Sw5-2, which determines resistance to TSWV, was found to be homozygous susceptible insert ignore into journalissuearticles values(rr); in 57 tomato lines, heterozygous resistant insert ignore into journalissuearticles values(Rr); in 27 tomato lines, and homozygous resistant insert ignore into journalissuearticles values(RR); in 5 tomato lines. No results were obtained in 7 tomato lines. For the marker insert ignore into journalissuearticles values(Mi23); that determines resistance to root-knot nematodes insert ignore into journalissuearticles values(Meloidogyne spp.);, 44 tomato lines were found to be homozygous susceptible insert ignore into journalissuearticles values(rr);, 11 tomato lines were heterozygous resistant insert ignore into journalissuearticles values(Rr);, and 35 tomato lines were homozygous resistant. No results were obtained for 6 tomato lines. It was concluded that the DNA molecular markers used are useful in determining resistance responses to TSWV, TYLCV and Meloidogyne spp. in tomato and can give reproducible and reliable results in a short time.
Keywords : Root Knot nematode, Tomato yellow leaf curl virus, Tomato spotted wilt virus, PCR, Molecular marker