Molecular Characterization of Gram Negative Isolated from Walnut (Juglans regia L.) Rhizosphere

Authors : Belgin ERDEM, Elif SEVİM, Ali SEVİM

Pages : 54-61

Doi:10.34248/bsengineering.957791

View : 14 | Download : 5

Publication Date : 2022-04-01

Article Type : Research Paper

Abstract :In this study, 10 Gram negative bacteria isolated from the rhizosphere soil of walnut insert ignore into journalissuearticles values(Juglans regia); orchard in the Kırşehir insert ignore into journalissuearticles values(Central Anatolia of Turkey); were identified at species level by classical and molecular methods. It was determined that 99% of phylogenetic similarities with bacterial isolates were closely related to the isolated Gram negative bacterial isolates. Ten isolates were morpho-biochemically and molecularly characterized and on the basis of 16S rDNA sequencing were identified as 3 Enterobacter cloacae, 3 Enterobacter ludwigii, 2 Escherichia coli, 1 Enterobacter hormaechei and 1 Plesiomonas shigelloides. Gram negative bacteria showed enzymes activities. Except for P. shigelloides and E. coli, other Gram negative bacteria showed lipase, protease, cellulase, amylase, gelatinase and pectinase activities at 4 °C and 28 °C. As a result, the current research suggests that strains isolated from walnut rhizosphere soil can be used as bio-fertilizing and bio-control agents.
Keywords : Gram negetive bacteria, 16S rRNA sequence, Juglans regia L, Rhizosphere