A New Spectrophotometric Method for Determination of Carvedilol from Tablet

Authors : Figen Erek, Işıl Aydın

Pages : 359-364

Doi:10.34248/bsengineering.1436216

View : 49 | Download : 61

Publication Date : 2024-03-15

Article Type : Research Paper

Abstract :In this study, a new spectrophotometric method was developed for the quantitative analysis of Carvedilol and the method was validated. The method depends on the reaction between the carvedilol and 1,2,5,8-tetrahydroxyanthraquinone in methanol to yield colored charge transfer complex giving maximum absorbance at 560 nm. For optimization of the proposed method, several parameters were investigated such as solvent type, reaction time, and quinalizarin concentration. The stoichiometry of colored charge transfer complex was found to be 2:1(reagent: drug) by Job’s method. Beer’s Law is obeyed in the concentration range of 0.5-60 µg/mL with 0.9986 correlation efficient. Limit of detection (LOD) and limit of quantifiqation (LOQ) were found 0.147 µg/mL, 0.491 µg/mL, respectively. The proposed method can be successfully applied pharmaceutical formulation.
Keywords : Charge transfer complex, Jobs method, Molecular absorption spectroscopy