İlaç Hammaddesi Olarak Sentezlenen Elektrofilik ve Nonelektrofilik Fenolik Schiff Bazlarının Umu Test Sistemi ile Genotoksik Aktivitesinin Araştırılması

Authors : Emre CEYHAN, Ayşe MERCANGÖZ, Nimet KARAKOÇ, Sevim BİLGİÇ

Pages : 0-0

View : 16 | Download : 8

Publication Date : 2010-04-01

Article Type : Research Paper

Abstract :In this work, we investigated, through a short-term genotoxicity experiment, the umu-test, the mutagenic activities of a group of phenolic Schiff bases, namely Material A 2-insert ignore into journalissuearticles values(N-phenyliminomethyl);-phenol, Material B 2-insert ignore into journalissuearticles values(N-p-bromophenyliminomethyl);- phenol, Material C 2-insert ignore into journalissuearticles values(N-p-methylphenyliminomethyl);-phenol and Material D 2-insert ignore into journalissuearticles values(N-panilinephenyl iminomethyl);-phenol, the substances which we synthesized, in the chemistry laboratories of our university, to form the raw materials of medicines. The umu-test method is a colorimetric bacterial test system and has been developed on the basis of SOS response induced by DNA damage. Materials A, B, C and D and the experimental materials prepared in 5 different dose levels by dissolving these materials in DMSO have not increased the galactosidase enzyme activity. When calculated with respect to the enzyme unit calculation formula, results seemed to be not larger than the unit value of the positive mutagenic material 4NQO. Results have also been evaluated statistically.
Keywords : Umu, genotoxicity, mutagenicity, phenolic Schiff base