The effects of hydrogen peroxide and mitochondria on human breast cancer cells

Authors : Zehra ÇİÇEK

Pages : 109-116

Doi:10.17826/cumj.1187258

View : 14 | Download : 7

Publication Date : 2023-03-31

Article Type : Research Paper

Abstract :Purpose: It was aimed to evaluate the effect of hydrogen peroxide insert ignore into journalissuearticles values(H2O2);, mitochondria isolated from human breast cancer cells insert ignore into journalissuearticles values(MCF-7); and human mesenchymal stem cells insert ignore into journalissuearticles values(IMSCs); on MCF-7 cell proliferation in this study. Materials and Methods: Mitochondria of IMSCs and MCF-7 cells were isolated according to the protocols specified in the kit and the protein amount of mitochondria was measured by the bicinchoninic acid method insert ignore into journalissuearticles values(BCA);. H2O2 doses insert ignore into journalissuearticles values(0-1000 µM);, mitochondria obtained from MCF-7 and IMSCs were transferred to MCF-7 cells and the viability was evaluated by tetrazolium salt insert ignore into journalissuearticles values(MTT); method. The cell percentages were calculated by measuring absorbance of the samples at 570 nm with a plate reader. Results: It was found that, ATP level of the mitochondria isolated from IMSCs was higher than MCF-7s. MCF-7 cell viability decreased significantly in 30 µM insert ignore into journalissuearticles values(%19);, 40 µM insert ignore into journalissuearticles values(%26);, 50 µM insert ignore into journalissuearticles values(%39);, 75 µM insert ignore into journalissuearticles values(%39);, 100 µM insert ignore into journalissuearticles values(%36);, 200 µM insert ignore into journalissuearticles values(%28);, 400 µM insert ignore into journalissuearticles values(%23);, 800 µM insert ignore into journalissuearticles values(%22); and 1000 µM insert ignore into journalissuearticles values(%23); H2O2 doses. The transfer of IMSCs mitochondria caused a tendency to decrease in cell proliferation, but no significance was detected. But, it was found that healthy mitochondria transfer with 10 µM and 1000 µM H2O2 reduced the proliferation of MCF-7s insert ignore into journalissuearticles values(respectively %14 and % 8);. Conclusion: It was determined that the transfer of healthy mitochondria isolated from IMSCs and H2O2 to MCF-7 is associated with proliferative processes, however transplantation of the healthy mitochondria into cancer cells is thought to be a promising new method in the treatment of the disease.
Keywords : kök hücre, kültür, proliferasyon