Modulatory effects of Hypericum perforatum extract on sciatic nerve injury-induced peripheral neuropathy: an experimental study on mice

Authors : Aylin SARIYILDIZ, Halil Mahir KAPLAN, Ergin ŞİNGİRİK, Erkan KOZANOGLU

Pages : 513-521

Doi:10.17826/cumj.1259761

View : 130 | Download : 208

Publication Date : 2023-07-02

Article Type : Research Paper

Abstract :Purpose: The effect of Hypericum perforatum insert ignore into journalissuearticles values(HP);, which is a medicinal plant, on sciatic nerve injury-induced peripheral neuropathy has been less studied so far. The current experimental study aimed to investigate the neuroprotective and antinociceptive effects of Hypericum perforatum insert ignore into journalissuearticles values(HP); extract on sciatic nerve injury-induced peripheral neuropathy in mice. Materials and Methods: In the present study, 18 Balb/C albino mice were allocated equally into three groups. The first group was determined as controls, and no procedure was performed on these mice. Neuropathy was generated by the partial sciatic nerve ligation method on mice allocated to the second and third groups. Mice in the third group received HP extract at a dose of 70 mg/kg per day for fourteen days. Nociception insert ignore into journalissuearticles values(cold allodynia); was evaluated using the cold plate test at the end of the experimental period. Tumor necrosis factor –αlpha insert ignore into journalissuearticles values(TNF-α); and interleukin-6 insert ignore into journalissuearticles values(IL-6); in plasma; inducible nitric oxide synthase insert ignore into journalissuearticles values(iNOS);, phospholipase A2, cyclooxygenase-2 insert ignore into journalissuearticles values(COX-2);, nuclear factor-kappa B insert ignore into journalissuearticles values(NF-κB);, caspase-3, Bcl-2, and Bax levels in sciatic nerve were measured by enzyme-linked immunosorbent assay test. Results: Cold plate latencies insert ignore into journalissuearticles values(sec); of the neuropathy + HP, neuropathy, and control groups were 8.33 ± 0.67, 5.17 ± 0.60, and 13 ± 0.73, respectively. Plasma TNF-α, IL-6 levels, and sciatic nerve iNOS, COX-2, NF-κB, caspase-3, and Bax levels were significantly decreased after HP supplementation. Bcl-2 levels of the neuropathy + HP, neuropathy, and control groups were 9.92 ± 0.71, 5.37 ± 0.53, and 13.65 ± 0.68, respectively. Conclusion: HP has improved oxidative, inflammatory, and apoptotic responses, as well as cytokine levels in plasma and sciatic nerves of mice. It has been concluded that HP provided neuroprotective, anti-inflammatory, and antinociceptive effects in experimental mice with sciatic nerve injury models, which is suggested to guide future studies on neuropathic pain management.
Keywords : Sitokin, Hypericum perforatum, nöroinflamasyon, nöropatik ağrı, siyatik sinir hasarı