Association of Kruppel-like factor 2 polymorphisms with the risk of coronary artery disease

Authors : Ulaş DEĞİRMENCİ, Metin YILDIRIM, Merih AKKAPULU, Ali YALIN, Dilek çiçek DİLEK ÇİÇEK, Ragıp Onur ÖZTORNACI, Bahar TAŞDELEN, Serap YALIN

Pages : 583-592

Doi:10.17826/cumj.1283236

View : 41 | Download : 116

Publication Date : 2023-07-02

Article Type : Research Paper

Abstract :Purpose: Coronary artery disease is defined as complications that develop in proportion to the prevalence of ischemia due to occlusion of the coronary arteries and the resulting cell death. The development of atherosclerosis is significantly influenced by endothelial cell dysfunction. Kruppel-like factor 2, a transcription factor, has been shown to regulate critical biological events in endothelial biology, such as vascular tone, migration, proliferation, vasoreactivity, and angiogenesis. In our study, it was aimed to clarify the relationship between coronary artery disease and Kruppel-like factor 2 protein levels and C1239A polymorphisms. Materials and Methods: 191 individuals who underwent coronary angiography at Mersin University Faculty of Medicine, Department of Cardiology, Health, Research and Application Center were included in the study. Measurements of serum fasting blood glucose, HDL cholesterol, total cholesterol, and triglyceride levels were performed on the AU5800 insert ignore into journalissuearticles values(Beckman Coulter, United States); autoanalyzer. Serum LDL levels were calculated using the Friedwald equation. Serum Kruppel-like factor 2 protein levels were measured by sandwich enzyme-linked immunoassay method on the Multiskan GO insert ignore into journalissuearticles values(Thermo Scientific, Finland); device. Kruppel-like factor 2 C1239A variations were detected on the Applied Biosystem VIIA™ 7 Real-Time PCR insert ignore into journalissuearticles values(Life Technologies Co., United States); device by TaqMan® single nucleotide polymorphism insert ignore into journalissuearticles values(SNP); genotyping method. Results: Men had a 3.8-fold higher risk of CAD than women. insert ignore into journalissuearticles values(Odd’s ratio 3.83, 95% Confidence interval 1.98-7.39; p<0.001);. Kruppel-Like Factor 2 protein levels were not significantly different amongst the groups. Kruppel-like factor 2 C1239A polymorphisms were not associated with coronary artery disease. Conclusion: More comprehensive studies with larger sample groups including other gene regions and meta-analysis studies are needed to determine the relationship between Kruppel-like factor 2 gene polymorphisms and coronary artery disease.
Keywords : Koroner arter hastalığı, ateroskleroz, endotel, kruppel benzeri faktör 2, tek nükleotid polimorfizmi