Thymoquinone reduces methotrexate-induced heart damage: a histopathological study in rats

Authors : Ayşegül Burçin YILDIRIM, Emin KAYMAK, Tayfun CEYLAN, Ali AKIN, Nurhan KULOĞLU, Meryem SAYAN, Necla DEĞER, Esra ÖNAL, Derya KARABULUT

Pages : 844-851

Doi:10.17826/cumj.1314101

View : 33 | Download : 71

Publication Date : 2023-09-30

Article Type : Research Paper

Abstract :Purpose: The study aimed to evaluate the effect of thymoquinone on cardiac tissue in MTX-induced cardiac toxicity in rats with various parameters. Materials and Methods: Group I insert ignore into journalissuearticles values(n:8); was administered intraperitoneal saline for 10 days. Intraperitoneal olive oil was applied to Group II insert ignore into journalissuearticles values(n:8); for 10 days. Group III insert ignore into journalissuearticles values(n:8); was administered a single dose of 20 mg/kg Methotrexate insert ignore into journalissuearticles values(MTX); insert ignore into journalissuearticles values(500 mg/20 ml); intraperitoneally on the 1st day of the experiment. Since Methotrexate was in liquid form, no solvent was used. Group IV insert ignore into journalissuearticles values(n:8); received 10 mg/kg Thymoquinone insert ignore into journalissuearticles values(THQ); intraperitoneally for 10 days. Group V insert ignore into journalissuearticles values(n:8); insert ignore into journalissuearticles values(MTX: insert ignore into journalissuearticles values(20 mg/kg single dose intraperitoneally on the 1st day);; THQ: 10mg/kg i.p. administered for 10 days. At the end of the experimental period, the rats were sacrificed for analysis of heart tissue. The structure of heart tissue was evaluated by haematoxylin-eosin staining. Immunohistochemically, connexin-43, HSP90, and HIF-1α antibodies were stained. The results were analysed statistically. Results: According to our results, thymoquinone has a positive effect on the expression of Cx43, one of the proteins providing transmission in the intercalary discs, HSP90, one of the chaperones in the cell, and HIF-1α expression against MTX toxicity and provides a significant improvement by showing a cardioprotective effect histopathologically. Conclusion: THQ could be considered a crucial cardioprotective phytochemical against MTX cardiotoxicity.
Keywords : Konneksin 43, HSP90, HIF 1α, metotreksat, timokinon