7-Methoxyflavone and 7-Hydroxy-4`-nitroisoflavone confer ameliorative effects to human erythrocytes exposed to oxidative damage

Authors : Mucip GENİŞEL, Sedat AYDIN

Pages : 6-11

View : 17 | Download : 4

Publication Date : 2021-05-30

Article Type : Research Paper

Abstract :Human erythrocytes are in constant motion within the blood, therefore, they contact inevitably with factors such as different toxic substances, drugs, reactive oxygen species. This situation may lead to a decline in the life span of erythrocytes by disrupting their metabolism. In the present study, the effect of 7-Methoxyflavone insert ignore into journalissuearticles values(MP); and 7-Hydroxy-4`-nitroisoflavone insert ignore into journalissuearticles values(HNF); on erythrocytes exposed to oxidative damage with hydrogen peroxide insert ignore into journalissuearticles values(H2O2); application has been investigated. The possible mitigating effect on the oxidative damage of these flavonoid derives have been tried to be revealed with biomarkers such as total superoxide dismutase insert ignore into journalissuearticles values(SOD);, manganese SOD insert ignore into journalissuearticles values(MnSOD); and cupper-zinc SOD insert ignore into journalissuearticles values(CuZnSOD);, catalase insert ignore into journalissuearticles values(CAT); and lipid peroxidation. H2O2 application caused serious decreases in TSOD and CAT enzyme activities as well as the band intensities of MnSOD and CuZnSOD isoenzymes in erythrocytes. In addition, this H2O2 increased critically the oxidative products of lipid peroxidation. MP and HNF treatment significantly reduced the level of lipid peroxidation by increasing the antioxidant enzymes activities of erythrocytes in oxidative stress. As a result, it has been revealed that MP and HNF contributes to the attenuation of oxidative damage in human erythrocytes with its own antioxidant effect and/or by promoting antioxidant enzymes.
Keywords : Human erythrocyte, oxidative damage, 7 Hydroxy 4` nitroisoflavone, 7 Methoxyflavone, antioxidants, flavonoid