Effect of GA3 concentrations in basal medium on embryos germination of Cleopatra mandarin x carrizo citrange and Cleopatra mandarin x Flying Dragon

Authors : Ertugrul Turgutoglu, Senay Kurt, Gulay Demir

Pages : 47-49

View : 18 | Download : 5

Publication Date : 2015-01-01

Article Type : Research Paper

Abstract :We investigated the viability and in vitro germination of pollen in seven lemon varieties insert ignore into journalissuearticles values(Citrus limon insert ignore into journalissuearticles values(L.); Burm f.); insert ignore into journalissuearticles values(‘BATEM Sarısı’, ‘BATEM Pınarı’, ‘Interdonato’, ‘Kütdiken’, ‘İtalyan Memeli’, ‘Meyer’ and ‘Lamas’);. The viability was tested with TTC insert ignore into journalissuearticles values(2, 3, 5–triphenly tetrazolium chloride); and in vitro germination was tested with agar–plate to estimate pollen viability and germination in these varieties. Additionally, effect of different sucrose concentration levels insert ignore into journalissuearticles values(5%, 10%, 15%, 20% and 25%); for in vitro pollen germination was observed. The results indicated that pollen of ‘Meyer’ had the highest viability with 86.74%. The pollen of ‘Lamas’ and ‘Meyer’ varieties had the highest in vitro germination with 39.77% and 39.04%, respectively. This study results showed that 20% and 25% sucrose concentrations in the agar plates had the highest germination with 36.16% and 32.12%, respectively
Keywords : lemon, pollen, viability, germination, sucrose