Floral and Molecular Characterization of Gamma Rays Induced Mutants in Oil-Bearing Rose (Rosa damascena Mill.)

Authors : Hasan BAYDAR, Ufuk ÇELİKKOL AKÇAY, Soner KAZAZ, Nilgün GÖKTÜRK BAYDAR

Pages : 21-33

View : 29 | Download : 4

Publication Date : 2021-01-01

Article Type : Research Paper

Abstract :This research was aimed to create the genetic variations for the selection of oil-bearing rose genotypes with the desirable floral features. For this reason, gamma rays at doses of 0, 100 and 200 Gy of radioactive Cobalt-60 were applied to oilbearing rose seeds to create genetic variations. Finally, the floral and molecular characterization of oil-bearing rose plants derived from non-irradiated and irradiated seeds were carried out. A total of 48 genotypes including 17 from control insert ignore into journalissuearticles values(0 Gy);, 18 from M100 insert ignore into journalissuearticles values(100 Gy); and 12 from M200 insert ignore into journalissuearticles values(200 Gy); together with the parental species R. damascena were used as genetic materials. Genetic analysis was performed by using 20 SSR primer pairs. After the electrophoresis, the bands were displayed in TIF format with the aid of the Biolab UV Tech gel imaging system. The genotypes and mutants grown from irradiated and non-irradiated seeds were significantly different with flower colours from white to dark pink and petal numbers from 5 to 100. These results also showed that the seeds in the open-pollinated flowers of oil-bearing rose had mostly heterozygous allele genes governing the floral traits. The efficacy of the SSR primers used to identify mutations was different. RA003a and RA034a primers were found to be more effective in mutation screening in oil-bearing rose genome. It was understood that the major effects of the gamma-ray irradiation were on large-scale chromosomal breaks or deficiencies. In conclusion, oil-bearing rose seeds with or without gamma-ray irradiation would be a huge selection source to breed novel varieties.
Keywords : Rosa damascena Mill, mutation breeding, gamma ray, SSRs