In vitro Plant Regeneration Efficiency from Different Explants of Local Sainfoin Ecotype (Onobrychis sativa)

Authors : Mehmet Burak TURGUT, Muhammet Çağrı OĞUZ, Burak ÖNOL, Cengiz SANCAK

Pages : 101-107

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Publication Date : 2022-07-31

Article Type : Research Paper

Abstract :Local populations and ecotypes are important genetic resources with genetic diversity. It is possible to protect threatened genotypes and increase biodiversity with in vitro tissue culture techniques. Explant type is of great importance among the factors affecting the success of in vitro regeneration and callus induction. In this study, Sainfoin seeds belonging to the Gözlü ecotype were used as experiment material. MS media were prepared with TDZ and combinations of IBA-NAA. Callus induction and shoot regeneration rates of hypocotyl, leaf and cotyledon explants were determined in the sainfoin ecotype. According to the results of the study, the best callus formation and shoot regeneration were obtained from the hypocotyl explant in an MS medium containing 2 mg/L TDZ. In the present study, important results were obtained that will contribute to the regeneration studies of local ecotypes and wild species, genetic transformation and increasing genetic diversity.
Keywords : In vitro regeneration, callus induction, regeneration efficiency, sainfoin ecotype