Diversity Analysis for Drought Tolerance in Pearl Millet Inbred Lines using SSR Markers

Authors : Jagdeep Singh, Ashok K Chhabra, Rishi K Behl, Akshay K Vats, Pooja Malik

Pages : 36-51

View : 19 | Download : 17

Publication Date : 2024-02-01

Article Type : Research Paper

Abstract :50 pearl millet genotypes (inbred lines) were grown in RBD design at two contrasting locations in Haryana, India including one at CCSHAU Hisar and another at RRS, Bawal. Genomic DNA was isolated from leaves of 2-3 weeks old plants using CTAB extraction method. The SSR diversity data was used to determine the genetic relationship among the fifty genotypes. A similarity matrix was first made using SIMQUAL subprogram of software. The dendrogram was then constructed based on the simple matching coefficient using SAHN sub-program. The SAHN sub-program uses UPGMA algorithm to perform cluster analysis. Out of 50 SSRs used for identification of single marker analysis 9 SSRs were found polymorphic for further checking their behaviour on known drought tolerant (HTP 93-37, HTP 03/13-901-1) and drought-sensitive inbreeds (HM S33B, HMS 42B) and were used for amplification of DNA. SSRs showed amplification for all genotypes and thus confirmed in other genotypes for the study of drought in pearl millet genotypes. Primers amplified a total of 237 alleles which varied from 2 to 8 with a mean of 4.54 alleles per locus. The overall size of PCRamplified products ranged from 140 bp (PSMP 2271) to 810 bp (ICMP 10). Polymorphic information content (PIC) value ranged from 0.326 (PSMP2201) to 0.89 (XCUMP 009) with an average of 0.579. 50 Inbred lines based on SSR marker polymorphism data were resolved into 11 diverse clusters two genotypes HTP 9337 and HMS 43B were failed to fall into any cluster. Based on SSR markers and morphophysiological data two inbred lines (HTP 93-37, HTP 03/13-901-1) appeared drought tolerant which may be used for hybrid development program.
Keywords : Inbred, drought tolerant, polymorphism, genotypes, SSR markers