Determination of Resistance to Tomato Yellow Leaf Curl Virus by Molecular Methods in Pink Beef Tomatoes

Authors : Mine Bulut, Sevinç Tekin, Elif Ürün Köksalan, Veysel Aras

Pages : 52-58

View : 23 | Download : 14

Publication Date : 2024-02-01

Article Type : Research Paper

Abstract :In both public and private tomato breeding projects, marker assisted selection (MAS) for disease resistance is frequently used. In tomato molecular breeding programs, the development and application of molecular markers have been extensively pursued, particularly for disease resistance to enable the selection of a single resistance gene or a combination of multiple resistance genes. Tomato Yellow Leaf Curl Virus (TYLCV) is one of the most dangerous viruses affecting tomato production and growth worldwide. Using resistant cultivars is the most effective and eco-friendly way to combat TYLCV. In this study, the TYLCV was tested against 155 genotypes of pink beef tomatoes utilizing the MAS (MarkerAssisted Selection) technique. Resistance against TYLCV was determined with the SCAR (P6-25) primer developed in connection with the Ty-3 gene. 42 pink tomato genotypes were determined to be sensitive (rr), 99 to be heterozygous resistant (Rr), and 8 to be homozygous resistant (RR) to TYLCV as a consequence of MAS testing. Furthermore, no molecular marker was found in any of the six pink beef tomato genotypes. These findings suggested that P6-25 (SCAR) primers could be used successfully in breeding studies to identify disease resistance.
Keywords : Tomato, MAS, TYLCV, resistance breeding