Investigation of CRISPR anti-phage systems of Lactobacillus plantarum from pickled olives

Authors : Asiye Esra EREN, İhsan YAŞA, Nazenin EFTEKHARİ

Pages : 159-168

View : 14 | Download : 8

Publication Date : 2020-12-31

Article Type : Research Paper

Abstract :Lactobacillus plantarum is the most commonly used microorganism in industrial food fermentations. The multidrug resistance, bacteriocin production, various enzyme activities, probiotic properties and the resistance of the strain against bacteriophages, are important for application in industrial field. Microorganisms have developed various survival strategies in the evolutionary process. Genomic antiphage role of Clustered Regularly Interspaced Short Palindromic Repeats insert ignore into journalissuearticles values(CRISPR-Cas); is one of the defense strategies. This is an RNA-based immune system involved in the collaboration of CRISPRrelated insert ignore into journalissuearticles values(cas); genes with DNA sequences of foreign genetic elements inserted between the repeated sequences. In this study, a rapid diagnosis of L. plantarum was carried out among 59 pickled olives by Real-Time Polymerase Chain Reaction insert ignore into journalissuearticles values(PCR); with species-specific probes and primers. Twenty-five isolates were identified as L. plantarum. The presence of CRISPR-Cas loci of the genomic anti-phage system in these identified species was investigated using DNA based and bioinformatics methods such as PCR, sequencing, and molecular software. Specific primer design for L. plantarum CRISPR arrays was performed. CRISPR loci were detected in 14 L. plantarum strains via classical PCR method. The results were analyzed using NCBI-Blast, CRISPRFinder databases. In one of the isolates, 6 repeats, and 5 spacers nucleotide arrays were found. CRISPR-related proteins insert ignore into journalissuearticles values(Dead/Deah box helicases); were detected in remaining isolates.
Keywords : pickled olives, L plantarum, Taqman probe, Crispr cas, anti phage system, CrisprFinder