BLINDED COMPARISON IN IDENTIFICATION OF YEASTS: MYCOLOGIST+CONVENTIONAL METHODS VS BD PHOENIXTM

Authors : Ali Korhan SIĞ, Muharrem NASLI

Pages : 127-133

Doi:10.48176/esmj.2023.119

View : 42 | Download : 41

Publication Date : 2023-07-28

Article Type : Research Paper

Abstract :Objective: Epidemiology of fungal infections has changed in the last years due to various reasons. Accurate identification of causative fungi is crucial to guide treatment. The aim of this study is to compare yeast identifications of BD PhoenixTM 100 System insert ignore into journalissuearticles values(Becton Dickinson, USA); device and mycologist+conventional methods. Materials and Methods: Various fungal cultures of years 2020-2023 in Balıkesir Atatürk City Hospital were included. Isolated yeasts were double-blindly evaluated in identification by conventional methods insert ignore into journalissuearticles values(colony morphology, germ tube testing, urease positivity, cycloheximide resistance, cornmeal tween 80 agar testing, thermotolerance, ascospore existance, producing metallic green sheen with eosin-methylene blue agar, existance of capsule formation and observation for pellicle formation on sabouraud dextrose broth); and BD PhoenixTM 100 System insert ignore into journalissuearticles values(Becton Dickinson, USA);. Bulgular: A total of 353 yeast isolates with Candida albicans that had the highest amount insert ignore into journalissuearticles values(n=142); were included. Candida albicans, Candida parapsilosis complex, Candida glabrata complex, Candida tropicalis, Candida dubliniensis, Candida kefyr, Saprochaete capitata, Candida krusei, Candida lipolytica, Candida inconspicua and Trichosporon asahii were identified in species-level by both methods, while Rhodotorula spp. and Saccharomyces spp. were compatibly identified in genus-level by conventional method, but in species-level by automated method. Conventionally identified six Candida spp. strains were resulted as Candida sake, Candida zeylanoides, Candida lambica, Candida guilliermondii complex by automated system. Five isolates could not be identified by both methods and two isolates were defined as S.capitata by only the system. In overall, 92.9%, 99.4% and 100% compatibility were observed in species-level, genus-level and in top 5 species, respectively Results: Species-level identification takes an important place in order to guide treatments and laboratories should optimize their diagnostic capacities. Although conventional methods are generally unsuccessful in identification of rare species, total compatibility was observed for common species. Laboratories should evaluate their patient profile and capacities to choose either one of them.
Keywords : fungal enfeksiyonlar, mantarlar, mayalar, tanımlama, Candida