Selective and sensitive electrochemical sensing of serotonin in human blood serum by means of electrochemically treated pencil graphite electrode

Authors : Ali Özcan

Pages : 551-562

Doi:10.18038/btda.63537

View : 14 | Download : 8

Publication Date : 2016-10-03

Article Type : Research Paper

Abstract :Electrochemical determination of serotonin insert ignore into journalissuearticles values(SER); in blood serum was investigated by electrochemically treated pencil graphite insert ignore into journalissuearticles values(ETPG); electrode for the first time. The electro-catalytic effect of ETPG shifted the oxidation potential of SER towards more cathodic direction insert ignore into journalissuearticles values(40 mV);. The high surface area of ETPG together with its electro-catalytic effect increased the oxidation peak current values tremendously insert ignore into journalissuearticles values(forty times); according to bare PG. Moreover, the ETPG electrode allowed the oxidation of SER and uric acid insert ignore into journalissuearticles values(UA); at separate potential values that was not possible in the case of bare PG. It was observed that the interference effect of UA, which was a major component of blood serum, was negligible at pH value of 1.6. The ETPG showed good performance for the determination of SER with a linear range between 0.005 μM and 1.0 μM. The relative standard deviation of ten repetitive measurements of 1.0 μM SER was calculated as 2.8 % indicating highly reproducible response of ETPG electrode. The detection limit was calculated as 1.0 nM insert ignore into journalissuearticles values(S / N = 3);. The analytical application of the proposed method was successfully tested in blood serum samples and it was compared with a previously published HPLC method.
Keywords : Serotonin, Electrochemically treated pencil graphite, Uric acid, Blood serum, Voltammetry