Multiresidue chromatographic method for the determination of antibiotic residues in honey by high-performance liquid chromatography with DAD detection

Authors : Bouchra RACHİD, Ali JABER, Edmond CHEBLE

Pages : 157-161

Doi:10.46239/ejbcs.1104606

View : 7 | Download : 5

Publication Date : 2022-12-30

Article Type : Research Paper

Abstract :Clandestinely, consumers may be exposed to antibiotic insert ignore into journalissuearticles values(ATB); residues in honey, which could pose a health concern. For the first time, the simultaneous determination of Florfenicol insert ignore into journalissuearticles values(FF);, Penicillin G insert ignore into journalissuearticles values(PG);, and Tetracycline is described in this paper. The multiresidual method was developed and optimized using high-performance liquid chromatography insert ignore into journalissuearticles values(HPLC); coupled to a diode array detector insert ignore into journalissuearticles values(DAD);. These ATBs were separated on a C18 analytical column after a cleanup process followed by solid-phase extraction insert ignore into journalissuearticles values(SPE);. For the first time, the chromatographic conditions were perfected. After the method validation process, the method was used to assess ATB residues in four Lebanese honey samples. ATBs were separated in less than 15 min with an isocratic elution using a mixture of 80 % potassium dihydrogen phosphate aqueous solution, and 20 % acetonitrile. The UV detection was performed at 350 nm for TC, 224 nm for FF, and 230 nm for PG. The proposed method was linear insert ignore into journalissuearticles values(R2 ≥ 0.996); within the concentration ranges of 0.7-17.5 mg.Kg 1 for the three compounds. Both intra- and inter-day precision, expressed as RSD, were ≤15 %. The method was subsequently successfully applied to analyze examined ATB residues in honey samples collected from Lebanese beekeeping. The method described could be a valuable tool to conduct a comprehensive survey of honey samples produced in Lebanon, especially in the lack of serious national oversight.
Keywords : Multiresidue, Antibiotics, HPLC DAD, Honey, SPE