DNA barcoding of Campanula choruhensis Kit Tan & Sorger Endemic to Artvin (Türkiye)

Authors : Hayal Akyıldırım Beğen, Özgür Eminağaoğlu, Melahat Özcan

Pages : 10-18

Doi:10.31195/ejejfs.1401687

View : 124 | Download : 163

Publication Date : 2024-02-25

Article Type : Research Paper

Abstract :DNA barcoding is the method of description of species based on gene diversity. In current studies, registration, genetic identification and protection of especially endemic plant species are carried out by DNA barcoding techniques. Molecular studies are based on the amplification and sequencing of the barcode gene region by the PCR method. Campanula choruhensis Kit Tan & Sorger is endemic and widespread in Artvin, Erzurum and around Çoruh valley passing through it. Roots of this species are used in medicinal and aromatic studies. Intense roadworks and dam constructions are carried out in and around the distribution area of this species. This situation harms the habitat of the species and comes across with its extinction. In this study, the plastid barcode gene regions (trnK-rps16 and trnL-rpl32) of the C. choruhensis was sequenced from five populations. To make the identification of this species quickly and accurately, gene sequence compared with sequences of other related Campanula L. species. In addition, gene sequences and phylogenetic relations were given.
Keywords : Artvin, Campanula, DNA barkodlama, moleküler, tıbbi ve aromatik, Türkiye