Evaluation of municipal sewage sludge for Arbuscular mycorrhizal fungi inoculum production

Authors : Anju TANWAR, Ajay SİNGH, Ashok AGGARWAL, Esha JANGRA, Sergio T. PİCHARDO

Pages : 343-353

Doi:10.18393/ejss.972157

View : 15 | Download : 6

Publication Date : 2021-10-01

Article Type : Research Paper

Abstract :This experiment was carried out to assess the effect of soil amendment with different concentrations of municipal sewage sludge insert ignore into journalissuearticles values(SS); as a substrate on inoculum production of two selected arbuscular mycorrhizal fungi insert ignore into journalissuearticles values(AMF); i.e., Glomus mosseae and Acaulospora laevis. The experiment was a 4 × 5 factorial design with four hosts including, maize insert ignore into journalissuearticles values(Zea mays L.);, lemon grass insert ignore into journalissuearticles values(Cymbopogon nardus insert ignore into journalissuearticles values(L.); Rendle);, palmarosa insert ignore into journalissuearticles values(Cymbopogon martini insert ignore into journalissuearticles values(Roxb.); Wats.); and Sesbania insert ignore into journalissuearticles values(Sesbania aculeata Poir.); and the following five SS concentrations 1); no substrate, 2); 25 g, 3); 50 g, 4); 75 g and 5); 200 g pot–1); with five replications. After 90 days, the host roots and its rhizosphere soil were examined for fungal mycorrhization in terms of percent of root colonization and AMF spore quantification. Furthermore, we calculated the response of each host in terms of increase in plant height insert ignore into journalissuearticles values(cm);, root length insert ignore into journalissuearticles values(cm);, root, fresh shoots, and dry weight insert ignore into journalissuearticles values(g);. Mycorrhization pattern showed moderate to abundant intraradical mycelium, extraradical mycelium, vesicles, and arbuscules in all the host plants. This pattern varied with a change in the input level of SS. The 75 g treatment obtained the maximum mycorrhization of both the AMF, while the highest input level was detrimental to AMF and host plants` survival. Among the tested hosts, lemon grass and maize had a tremendous increment in G. mosseae and A. laevis inoculum respectively. Consequently, 75 g SS with lemon grass is the most compatible host–substrate combination capable of maximum G. mosseae and A. laevis spore production and root colonization and so far, highlights the significance of an alternative, cost–effective and affordable carrier medium that can be adopted by farmers as sustainable cultural practices for on farm AMF inoculum production.
Keywords : Acaulospora laevis, Agricultural waste, Glomus mosseae, Sludge utilization strategy, Lemon grass