In-Vitro Bioactivity Investigation of Sol-Gel Derived Alumina-Bovine Hydroxyapatite (BHA) Composite Powders

Authors : Azade YELTEN, Okşan Karal-Yılmaz, Zeynep Püren AKGUNER, Ayça BAL-OZTURK, Suat YILMAZ

Pages : 690-700

Doi:10.35378/gujs.541345

View : 15 | Download : 4

Publication Date : 2020-12-01

Article Type : Research Paper

Abstract :Alumina insert ignore into journalissuearticles values(α-Al2O3); and hydroxyapatite insert ignore into journalissuearticles values(HA, Ca10insert ignore into journalissuearticles values(PO4);6insert ignore into journalissuearticles values(OH);2); are well-known for being clinically successful bioceramic materials. In this work, in-vitro biological characterization of the sol-gel alumina-bovine hydroxyapatite composite powders was realized. Alumina powders were synthesized through the sol-gel process. First, boehmite insert ignore into journalissuearticles values(AlOOH); sol was prepared utilizing aluminium isopropoxide insert ignore into journalissuearticles values(Alinsert ignore into journalissuearticles values(OC3H7);3, AIP); as the starting precursor. Bovine hydroxyapatite insert ignore into journalissuearticles values(BHA); powders, which can be defined as naturally derived calcium phosphate powders were added as 10, 20, 30, and 50% wt. of AIP to each AlOOH sol. Homogeneous dispersion of the BHA powders in the AlOOH sol was managed due to employing Na-alginate as a kind of thickener. Gelation of the AlOOH-BHA mixtures was carried out at 110 ºC for 3h. After drying, AlOOH-BHA mixtures were heat-treated at 1300 ºC for 2h. Chemical, microstructural, thermal, and physical properties of the precursors/process products were characterized with X-Ray Diffraction insert ignore into journalissuearticles values(XRD);, Fourier Transform Infrared Spectroscopy insert ignore into journalissuearticles values(FT-IR);, X-Ray Fluorescence Spectroscopy insert ignore into journalissuearticles values(XRF);, Differential Thermal Analysis insert ignore into journalissuearticles values(DTA);, and Scanning Electron Microscopy - Energy Dispersive Spectroscopy insert ignore into journalissuearticles values(SEM-EDS); analyses. Indirect MTT assay was done to evaluate the biocompatibility of the Al2O3-BHA based biocomposite extracts using the L929 cell line. It is found that all Al2O3-BHA composite extracts with varying doses of 25% and 50% had no negative effect on the cell viability. In addition, % cell viability decreased with the increasing of the extract concentration. It can be concluded that the prepared Al2O3-BHA composites can be a good candidate for biomedical applications. 
Keywords : Alumina, Bovine Hydroxyapatite, Sol Gel Method, Biocomposites, In Vitro Bioactivity