The Study of Interaction Activity of Nickel (ll) Phthalocyanine Complex Bearing Tetra Substituted Phenoxy-3-Methoxybenzoic Acid Groups with DNA

Authors : Ali ARSLANTAS, Mehmet Salih AĞIRTAŞ

Pages : 1022-1032

Doi:10.35378/gujs.1089316

View : 167 | Download : 190

Publication Date : 2023-09-01

Article Type : Research Paper

Abstract :Nickel phthalocyanine complex containing 3-methoxybenzoic acid groups was acquired and specified by way of Fourier Transform Infrared, NMR and UV-Visble spectroscopy procedures. Interaction of PcNi with the DNA molecule was examined via electronic absorption spectra, fluorescence spectra, melting point, viscosity, and the electrophoresis technics, respectively. The interaction activity of PcNi against the DNA was examined by way of absorption spectra titrations and the fluorescence spectra, farther by conducting melting point, viscosity procedures in the buffer of a pH 7.02. The obtained outcomes from these methods demonstrated that PcNi indicated substantial binding affinity to the DNA via intercalating by Kb of 1.31 x 106 m-1. Further, the interacting activity of PcNi on the DNA was analyzed by which electrophoresis technique and this procedure indicated that PcNi complex exhibits strong binding affinity on the DNA.
Keywords : Calf thymus DNA, Phthalocyanines, UV Vis Spectroscopy, Electrophoresis, Melting Temperature