Anti-helmintic Activity of Myrtus communis L. Fruit Ethanol Extract on Nematodes of Caenorhabditis elegans and The Determination of Possible Active Ingredients

Authors : Hülya ÖZPINAR

Pages : 267-272

Doi:10.17350/HJSE19030000238

View : 17 | Download : 5

Publication Date : 2021-09-29

Article Type : Research Paper

Abstract :Myrtus communis L. insert ignore into journalissuearticles values(Myrtaceae); is a plant widely found in the Middle East and Mediterranean region, both naturally grown and cultivated. In our study, C. elegans nematodes were used as an anti-helminthic model and by determining the anti-helmintic effect of Myrtus communis L. fruit, it was aimed to find the active ingredients with possible anti-helmintic effects by GC-MS analysis. In our study, the anthelmintic effect of the ethanol extract of Myrtus communis L. fruit on C. elegans was investigated. Ethanol fruit extracts were added to NGM separately at final volume of 10 mg/mL, 5 mg/mL, 2.5 mg/mL, 1.25 mg/mL and experimental groups were formed. When the data were evaluated statistically, the difference between all experimental groups, negative control group and positive control group insert ignore into journalissuearticles values(pyrantel pamoate);, was significant insert ignore into journalissuearticles values(p<0.05);. According to the results of GC-MS analysis, the highest detected ingredients were 1,8-Cineole insert ignore into journalissuearticles values(3.46%);, Linalyl acetate insert ignore into journalissuearticles values(3.06%);, and Palmitic acid insert ignore into journalissuearticles values(2.90%);. It was observed that Myrtus communis L. fruit extract was more effective on C. elegans nematodes than pyrantel pamoate at the same concentration insert ignore into journalissuearticles values(5 mg/mL);, and 20 different active ingredients were determined according to our GC-MS analysis results. By studying the forms of these active ingredients separately or in different combinations, the ingredients and combinations with the strongest anti-helmintic activity can be determined, and it is thought that our findings may lead to further studies.
Keywords : Myrtus communis L, Caenorhabditis elegans, anti helmintic effect, 1 8 Cineole, Linalyl acetate, Palmitic acid