Determination and Speciation of Selenium in Pharmaceutical Samples, Spiked Veterinary Drug Samples with a Kinetic Catalytic Method

Authors : Esra BAĞDA

Pages : 185-191

Doi:10.17350/HJSE19030000296

View : 28 | Download : 33

Publication Date : 2023-06-30

Article Type : Research Paper

Abstract :In the present study a new kinetic catalytic method was developed for the sensitive determination of selenium in pharmaceutical formulations. The reaction between basic blue 3 insert ignore into journalissuearticles values(BB3); and sulphide catalysed by Se insert ignore into journalissuearticles values(IV); in the presence of acetic-phosphoric-boric acid buffer system. The decrease in the absorbance at 654 nm indicated the reduction of BB3. The presence of selenium insert ignore into journalissuearticles values(IV); accelerated the reaction rate. The method based on the linear correlation between amount of Seinsert ignore into journalissuearticles values(IV); and reaction rate. Under optimum conditions, the linear calibration range was found as 0.1-2.0 µg ml-1 by the fixed time method for the 0.5-5 min. The tolerance limits of various species was also studied. The interfering effect of some cations such as Cr3+, Fe3+, and Hg2+ was reduced with using cation exchange resin. The proposed method was successfully applied to spiked nasal spray and veterinary drug samples. Besides, total selenium, Se insert ignore into journalissuearticles values(IV); and Se insert ignore into journalissuearticles values(VI); speciation also conducted with reducing Se insert ignore into journalissuearticles values(VI); to Se insert ignore into journalissuearticles values(IV); with HCl in the synthetic mixtures.
Keywords : Selenium, pharmaceutical samples, veterinary drug