EFFECT OF TRIGONELLINE IN A MODEL OF APOPTOSIS IN RAT RETINA

Authors : Sevinç Arzu Postacı, Ali Dal, Ülkü Çeliker, Nevin İlhan, Ferda Dağlı

Pages : 349-365

View : 16 | Download : 36

Publication Date : 2024-08-31

Article Type : Research Paper

Abstract :Purpose: Determine the effectiveness of drop trigonelline and oral trigonelline (TG) as treatments for retinal apoptosis caused by N-methyl D-aspartate (NMDA) and to compare the effectiveness of TG drops with brimonidine tartrate (BT) drops. Methods: We randomly divided rats into six groups of seven each. We didn’t apply any action to Group1. On the first day of the experiment (FDE), we administered intravitreal phosphate buffered saline (PBS) to Group2, the negative control, without administering any treatment. We administered intravitreal NMDA to Groups 3, 4, 5, and 6 on the FDE. Group3 (the positive control) didn’t receive post-injection treatment. Group4 received oral TG, Group5 received TG drops; and Group6 received BT drops. All rats underwent histopathological and biochemical evaluations. Results: TUNEL labeling revealed no significant changes between Groups 1 (1.21±0.08) and 2 (1.27±0.11), whereas Group 3 (12.52±2.82) had considerably higher indices (p<0.001). Compared to Group3, drop TG (4.67±1.23) and drop BT (4.26±0.97) significantly reduced apoptosis (p<0.001). MDA levels peaked in Group3 (3.23±0.85), with substantial reductions in all treatment groups (p<0.05). SOD levels decreased considerably in Group3 (14.6±4.01) compared to group 1 (31.51±8.04, p<0.001), while oral TG, drop TG, and drop BT increased significantly (34.73±5.16, 38.46±3.60, 35.98±4.89, p<0.001). In Group3, iNOs protein expression increased by 32%, while the drop TG (~21%, p=0.037) and drop BT (~20%, p=0.04) groups showed substantial declines. Conclusion: Our study found that the retinal neuroprotective and antioxidant efficacy of TG drops was comparable to that of BT drops.
Keywords : Nöroproteksiyon, Retinal apopitozis, Trigonelline